Inflammasome Formation in THP-1 Cells

A client requested a custom, in vitro assay of inflammasome formation using THP-1 cells.

Goals:

• Establish culture conditions and protocol for inflammasome formation.
• Evaluate the effects of client’s therapeutic agent on inflammasome formation.

Cell Model

THP-1 Cells

Imaging Palette

Hoechst (nuclei)
Anti-human ASC

Treatments and Timelines

• Culture cells in 384-well, imaging microplates, following custom culture protocol.
• Treat with LPS or PMA 16hrs (overnight)
• On DIV2, treat appropriate wells with gramicidin A (activator of NLRP3 inflammasomes) for 40min.
• Fix and stain cultures for scanning and analysis.

REPRESENTATIVE IMAGES & QUANTITATIVE DATA