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In Vitro Oncology & Immuno-Oncology Assay Services

Our Ready-2-Go and Bespoke assay services combine high-content imaging with 2D and 3D disease-relevant models to deliver new insights into the effects of your therapeutic.


Cancer is a highly complex disease class characterized by aggressive, unchecked cellular proliferation leading to development of malignancies that, if left untreated, often result in severe, long-term tissue and organ damage or fatality. A high-content, imaging-based approach offers several advantages in the development of novel oncology therapeutics. In particular, the ability to multiplex readouts enhances the value of more straightforward assays, characterizing the impact of cancer therapeutics upon proliferation, apoptosis, necrosis, and mitochondrial health of 2D monocultures of immortalized cancer cell lines. Assessing therapeutic activity in more complex, 3D models, including cancer spheroids, patient-derived organoids, and co-culture models mimicking the tumor microenvironment, can address more complex mechanisms of action and ensure translatability to the clinic. Employing a high-content imaging approach can deliver highly impactful, multi-parametric data from these challenging yet relevant assays.

Assay Services

Explore our Ready-2-Go assays or contact us for bespoke options.

Ready-2-Go Assay Service

Cell Health

Evaluating the impact of drug candidates on cell proliferation and viability is essential to the success of any drug-discovery or drug-development campaign. Oncology-targeted therapeutics are generally designed to inhibit cancer cell proliferation and viability.

While straightforward cell proliferation assays may reveal inhibitory activity of therapeutics, overall cell health is multi-faceted, necessitating a multiplexed approach assessing cell viability, apoptosis, and necrosis to achieve a comprehensive picture of drug activity.

PhenoVista has developed a R2G Cell Health Assay Service, enabling robust, accurate, simultaneous quantitation of cell viability, apoptosis, necrosis, and proliferation using a homogeneous assay workflow with dye-based detection. This approach maximizes data quality by minimizing pipetting steps and eliminating wash steps that could exacerbate loss of dead or dying cells.

Ready-2-Go Assay Service

Mitochondrial Health

Mitochondria play a fundamental role in maintaining cellular health by converting sugars and oxygen into energy (ATP), using a process that relies on their membrane potential. Perturbations in mitochondrial function are detrimental to cell health and, therefore, are critical to monitor in any drug development campaign, whether desirable (e.g., in cancer treatment) or not (e.g., off-target toxicity).

PhenoVista’s R2G Mitochondrial Health Assay Service screens for changes in mitochondrial membrane potential using a fluorescent dye that accumulates within the mitochondria at a rate that depends upon its membrane potential. Additionally, we have integrated measurements of cell count (via nuclear staining) as well as necrosis (via a dead cell dye) to deliver a more comprehensive picture of how drug treatment impacts overall cell health.

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