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READY-2-GO
LIVER FIBROSIS
ASSAY SERVICE

Measure fibrotic induction and inhibition in primary hepatic stellate cells.

 BACKGROUND

Acute liver injury is typically repairable, but when there is repeated or chronic injury (e.g., alcohol abuse, hepatitis, and nonalcoholic fatty liver disease), attempts at repair can go awry, resulting in fibrosis. Despite the increasing prevalence of liver fibrosis, there are still no reliable treatments. Hepatic stellate cells (HSCs) are the major drivers of liver fibrosis, and emerging antifibrotic therapies aim to prevent/halt fibrotic induction in them.

KEY FEATURES

  • Uses human, primary cells in an in vitro model of liver fibrosis
  • Readouts that characterize intracellular & extracellular fibrosis indicators
  • Only 6-8 weeks from assay to report
  • Ability to bundle R2G assay services or transition to more complex, bespoke assay services with the same service provider

 THE CHALLENGE

A reliable, physiologically relevant, and scalable model of liver fibrosis requires a large investment of time and resources. Animal models are commonly used but have extremely limited throughput, and the translational gap between animals and humans is wide. Cell-culture models often use immortalized cells that do not mirror clinical outcomes, and those that use primary cells require extensive validation and optimization of each lot.

THE SOLUTION

Our Ready-2-Go Liver Fibrosis Assay Service is scalable to high-throughput screens, uses physiologically relevant cells, and features validated conditions to get you actionable data quickly. Our R2G service assesses the effects of your test articles in an optimized model that uses primary, human HSCs, circumventing the animals-to-humans, translational gap to save you time and money in your drug-discovery efforts.

PROCESS OVERVIEW

 

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KEY FEATURES

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Uses human, primary HSCs

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Get readouts that characterize intracellular  and extracellular fibrosis indicators

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Only 6-8 weeks from assay to report

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Option to bundle R2G Services or transition to Custom Services

ASSESS YOUR THERAPEUTICS' EFFECT ON FIBROTIC INDUCTION

HSCs are cultured using a specialized protocol and pre-treated with your test articles. Images are acquired and analyzed at various time points to measure fibrotic induction.

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AVOID SPECIES DIFFERENCES WITH PRIMARY, HUMAN HSCs

The use of animal models or immortalized cell lines limits clinical translatability. We use primary, human HSCs to circumvent the animals-to-human, translational gap, saving you time and money in your drug-discovery efforts.

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Ready-2-Go Liver Fibrosis
Assay Outline
Cells Primary, human hepatic stellate cells
Markers Nuclei, F-actin, α-SMA,
fibronectin (optional add-on)
Dosing 6 doses of each of your test articles
Positive Controls TGF-β-only (fibrotic inducer); Alk5i (Galunisertib) (inhibitor of fibrotic induction)
Negative Control Vehicle(s)

FEBRUARY_2024_v2

 

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RELATED READY-2-GO SERVICES

Browse related Ready-2-Go assay offerings. For custom assays, check out our Custom Services.

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Fibrotic induction and inhibition are measured in lung fibroblasts.
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LEARN MORE ABOUT
CUSTOM ASSAY SERVICES

Can't find what you need? Work with our team to design a custom assay.
EXPLORE CUSTOM OPTIONS