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APPLICATION NOTE

Cell Painting App Note

Learn more about how cell painting is used in drug-discovery applications and review an example using human, iPSC-derived hepatocytes.

OVERVIEW

Cell painting is a scalable, fluorescence imaging-based method that provides detailed, unbiased, comprehensive information about therapeutic candidates without a priori knowledge of their impact on cells. Cell painting is used in many drug-development campaigns and can drive forward your research through various applications, including mechanism of action (MOA) determination, toxicity profiling, and target identification.

To aid in our clients’ R&D programs, we specialize in developing in vitro, phenotypic screening assays that range from simple, monocultures to complex, multi-cell type models. We collaborate with our clients to design custom assays that will answer their most pressing questions.

HIGHLIGHTS

We outline a cell-painting study that used human, iPSC-derived hepatocytes, including

  • An overview of cell painting and its uses in drug discovery
  • Representative images
  • Uniform Manifold Approximation and Projection (UMAP)
  • Dendrogram and heat map

REPRESENTATIVE IMAGES

Representative images of stained hepatocytes. Treatment with various compounds induced clear, phenotypic differences.

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DOWNLOAD THE COMPLETE APP NOTE

  • Overview of cell painting and its uses in drug discovery
  • An example of a cell painting study using human, iPSC-derived hepatocytes
  • Data representative images, uniform manifold approximation and projection (UMAP), dendrogram, and heat map
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RELATED RESOURCES

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CASE STUDY

Screening a Compound Library in Cardiomyocytes with Cell Painting

Review a study where cell painting is used to compare the responses of cardiomyocytes treated with a library of proprietary compounds to that of a set of reference compounds.

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BLOG POST

Cell Painting in Preclinical Safety and Toxicology: Literally Taking a Closer Look

Cell Painting is a powerful imaging-based technique with immense potential for preclinical safety and toxicology assessments of therapeutic candidates.

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BLOG POST

Cell Painting: A Powerful Tool for Deciphering Mechanism of Action in Drug Development

Cell painting is the phenotypic profiling of cellular responses to compounds, enabling scientists to unravel elusive MOAs and expedite drug discovery.

CONTACT US

Let's discuss how we can help with your next study.
DOWNLOAD THE APPLICATION NOTE

CELL PAINTING APP NOTE

Overview

Learn more about how cell painting is used in drug-discovery applications and review an example using human, iPSC-derived hepatocytes.

Highlights

  • Overview of cell painting and its uses in drug discovery
  • Representative images 
  • Uniform Manifold Approximation and Projection (UMAP)
  • Dendrogram and heat map

 

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