Regulation of Adipogenesis Using a Small Molecule

A client requested a custom project to assess their therapeutic agent's ability to promote adipogenesis in primary, human, subcutaneous preadipocytes.

Goals:

• Establish in vitro model of adipogenesis to evaluate therapeutic efficacy.
• Compare client therapeutic agent’s ability to promote adipogenesis compared to reference treatments.

Cell Model

Primary, human, subcutaneous, preadipocytes

Palette

Hoechst (nuclei)
BODIPY (lipid droplets)
CellMask (whole cell)

Treatments and Timelines

• Culture cells in 384-well, imaging microplates
• Seed preadipocytes and culture for 3 days
• Add treatments and begin differentiation protocol.
• Change from differentiation medium to maintenance medium and culture until endpoint on DIV12.

REPRESENTATIVE IMAGES & QUANTITATIVE DATA