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READY-2-GO
LUNG FIBROSIS
ASSAY SERVICE

Predefined assay that uses primary, normal, human lung fibroblasts to generate an inducible model of pulmonary fibrosis. 

THE CHALLENGE

BIOLOGICALLY RELEVANT, CELL-BASED ASSAYS

Pulmonary fibrosis is a chronic and progressive disease whose prevalence and incidence have been increasing for decades. Its causes are variable and often undefined, and currently available treatments can extend survival but offer no protection from disease nor cure. Most in vitro pulmonary fibrosis assays struggle with achieving biological relevance, as they often use immortalized cell lines. Assays that use primary cells require lot-to-lot validation in the assay system, which quickly becomes costly in both time and money. Furthermore, many experimental set-ups use cell-culture formats that are not amenable to medium- nor high-throughput screening, which greatly limits the potential and efficiency of therapeutic-candidate identification and characterization. 

OUR SOLUTION

ASSAY USING PRIMARY, NORMAL, LUNG FIBROBLASTS

Our Ready-2-Go Lung Fibrosis Assay Service uses pre-validated assay conditions with primary, normal, human lung fibroblasts (NHLFs) that are cultured with a protocol that limits basal fibrotic induction to generate a model that is adaptable to medium- and high-throughput, therapeutic-candidates profiling.

APPLICABLE RESEARCH & DISEASE AREAS

Pulmonary fibrosis; fibroblast-to-myofibroblast transition (FMT) 

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ASSAY OUTLINE

NHLFs are seeded and cultured in 384-well plates. They are treated with TGF-β before the addition of reference inhibitor Alk5i (Galunisertib), along with your test articles. NHLFs are fixed and stained 48 and 72 hours after compound addition and imaged on a high-content microscope. These images are analyzed to obtain quantitative assessments of your drug candidates’ effects on fibrotic progression.

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