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CASE STUDY

Migration of Primary, Human Aortic Endothelial Cells

A client requested a custom project to measure the migration of primary, human aortic endothelial cells (AECS) on substrate-coated inserts.

 

Goals:
  • Establish an assay to measure the migration of AECs.
  • Compare the extent of cell migration onto substrate-coated inserts with uncoated control inserts.

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EXPERIMENTAL DESIGN

Cell Model

Primary, human aortic endothelial cells (AECs)

Palette

Hoechst (405 nm)
CellTrackerGreen

Treatments and Timelines
  • Timepoints: 24, 48, 72, 96 hours
  • Treatments:
    • Control: Uncoated insert
    • Substrate-coatedinsert

REPRESENTATIVE IMAGES & QUANTITATIVE DATA

REPRESENTATIVE IMAGES

Representative images of HSCs 72hrs after treatment with fibrotic inducer TGF-β.

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individual-data

 

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THP-1 Migration Assay

This study assesses whether a therapeutic candidate affects the migration of THP-1 cells.

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BROCHURE

Ready-2-Go Cell Health Handout

Learn about how PhenoVista's R2G Cell Health Assay Service can help you get multiplexed measurements of proliferation, apoptosis, and cell viability.

CONTACT US

Let's discuss how we can help with your next study.

DOWNLOAD THE CASE STUDY

Induction & Inhibition of Fibrotic State Using a Novel, Biologic Therapeutic

Overview

Review an example of a study designed to measure fibrosis induction and inhibition in hepatic stellate cells (HSCs).

Highlights

  • Overview of study objective and goals
  • Experimental design, including timelines and deliverables
  • Representative images and graphs
  • Study results

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