R2G Neuronal Apoptosis & Viability Assay Services

THE CHALLENGE

DIFFERENTIATING BETWEEN MECHANISMS OF CELL DEATH

Measuring neuronal viability is indispensible to assessing the safety and toxicity of drugs and therapeutic candidates targeting the CNS. Pathways and causes of neuronal death are complex and can manifest as apoptosis or necrosis - two distinct processes that both contribute to the general measurement of cell viability. Evaluating the impacts of drug candidates on these cell fates are required for moving therapeutic candidates into the clinic, but distinguishing between these processes can prove challenging. Furthermore, most cell-viability assays use immortalized neuronal lines, which greatly limits the physiological relevance of the data.

OUR SOLUTION

SIMULTANEOUSLY MEASURE APOPTOSIS AND VIABILITY IN HUMAN, iPSC-DERIVED GLUTAMATERGIC NEURONS

Our Ready-2-Go Neurotoxicity Assay Service uses multiplexed dyes to simultaneously measure apoptosis and viability in physiologically relevant cells – human, iPSC-derived glutamatergic neurons. This assay includes reference compounds that trigger apoptosis and excitotoxicity, which can lead to both apoptosis and necrosis. Combining this assay with our Ready-2-Go Neuronal Mitochondrial Health Assay Service provides you a general toxicity framework that can save you time and money in your drug-development campaign.

ASSAY OUTLINE

Human, iPSC-derived, glutamatergic neurons (Fujifilm CDI) are seeded and cultured in 384-well plates for 7 days. They are then treated with reference compounds that elicit apoptosis or excitotoxicity along with your test articles for 24 and 48 hours, at which points the neurons are fixed and stained. Cells are then imaged on a high-content microscope, and images are analyzed to provide you quantitative assessments of your compounds’ effects on neurotoxicity.