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Immunology & Inflammation Assay Services

Monitor more immune cell sub-populations with multiplexed imaging.

Overview

The human immune system plays a significant role in mitigating diverse disease pathologies, including response to foreign microbial infections and malignant cell growth. That said, when dysregulated, the immune system is directly linked to progression of autoimmune and inflammatory disease states, such as rheumatoid arthritis, multiple sclerosis, and psoriasis/psoriatic arthritis.

Myriad immune cell subtypes are generally involved in progressing the immune response to pathogens or autoimmune/inflammatory disease states. These may include T cells, B cells, macrophages, dendritic cells, and their various subtypes and polarization states.

A high-content, imaging-based approach offers several advantages in the development of novel immuno-therapeutics. Specifically, the ability to multiplex readouts enhances the value of more straightforward assays that characterize activity of therapeutic agents upon response of a single immune cell type, such as T-cell proliferation. This approach also enables assessment of therapeutic activity in more complex, physiologically relevant, in vitro models, such as primary immune cell co-cultures. Employing a high-content imaging approach can deliver highly impactful, multi-parametric data from these more challenging yet more relevant assays.

Ready-2-Go Assay Service

Lung Fibrosis

Chronic inflammation is characterized by repetitive cycles of injury and healing; and not surprisingly, is often associated with fibrosis since it is defined by the hyperproliferation of connective tissue during the healing process. Commonly used fibrosis assays are often either too low throughput or are poorly predictive of downstream success. Our Lung Fibrosis Assay Service uses primary, normal human fibroblasts (NHLF) cells cultured using a protocol that limits basal induction of fibrosis (which is often observed with routine, cell-culture procedures). This not only allows for enhanced control of fibrotic induction, but also affords a format amenable to higher-throughput therapeutics profiling.
Ready-2-Go Assay Service

Microglia Phagocytosis

Microglial cells play several key functional roles within the central nervous system.  However, their most critical function is in driving the immune response including engulfing foreign pathogens, acting as antigen-presenting cells (APCs), exerting cytotoxic effects, and mediating the inflammatory response. Owing to the diversity and importance of their functions, aberrant microglial function is implicated in myriad central nervous system diseasesTo date, assays developed for microglia research are typically conducted using immortalized and murine cell lines that do not adequately mirror human biology.   Consequently, we have developed our Ready-2-Go Microglia Phagocytosis assay service to address the need for a reliable, robust, biologically relevant in vitro system using primary human immune cells.

Unique Questions. Unique Solutions.