Cell Painting

Cell painting provides an open-ended, unbiased approach with which to learn what a test article does to your cell or disease model. Rather than focusing on a defined set of molecular markers, cell painting measures changes in key structural components including the nucleus, Golgi/ER, actin cytoskeleton and overall cell shape.

Phenovista offers cell painting screens in diverse cell models including human iPS-derived hepatocytes and cardiomyocytes. Phenovista has also applied this approach to 3D organoids.

Objective

Cast a wide net to explore the effect of diverse chemistries (or other libraries such as CRISPR) on various types of biological systems.

Design

The classical method uses a combination of fluorescent dyes to label subcellular structures including the nucleus, nucleoli, mitochondria, ER/Golgi and overall cell morphology.

Most commonly this approach is used on human cell lines such as U2OS; our adaptations have included applications using human iPS-derived cardiomyocytes, human primary hepatocytes and human gut enteroids.

Advantages

Scalable low-cost method to survey a wide range of treatments with little to no a priori bias towards specific molecular targets.

Cell Painting

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