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3D Organoid long

3D Cell Biology

The transition from 2D assays to 3D biology is much more than a one-dimensional problem. We can help you navigate this new space.

Overview

Our mission to incorporate the most relevant disease models into the services we provide has provided us ample experience working with 3D models. This allows our customers to access the benefits of 3D cultures, without the need to acquire the expertise and specialized equipment needed for these studies. The benefits of 3D culture go beyond their direct use to model various diseases in drug discovery. For example stem cells grown in 3D show faster rates of proflieration, which can shorten timelines sigfniciantly. One of the more common stages of drug discovery where 3D models has provided benefit is in safety and efficacy testing where drugs that may appear quite efficacious in 2D models completely drop out due to more difficult task of accessing penetrating a 3D tissue vs. 2D tissue

Bespoke Assay Service

Case Study #1: Ex-vivo Patient-Derived Xenograft Studies

Objective: Client was interested in evaluatng chemoetharpuetc agents, including stnadard o care and combinations in exvif NSCLC PDX models.

Design: PDX tumors were dissociated to establish 3D tumor fragments that can be cultured and tested in high throughput in vitro

Study Execution: We partnered with Champions Oncology to perform ex-vivo studies on their patient-derviced xenograft (PDX) models. The >1200 models represent a diverse set of tumor types with extensive cliniucal and morecular characterization and can be used for evaluation of therapeutiic treatment.. We generate 3D tumor fragments from the PDX tissue, for rapid, in vitro assessment of therapeutic candidates (small molecules, recombinant antibodies, immuno-therapies, and combination studies.

Bespoke Assay Service

Case Study #2: Gut Enteroids as a Model for Gut Injury Disease

Our client was interested in identifying compounds that coulud protentc against gut injufyr disaease and needed a model system that recapitulted intensitinal biology. DefiniGen could provide a stem-cell deriveed organiod for testing both stressts and protectecive effects of clienti molecules. 

Advantages: Organs form intestinal lumen, allowing fo rmodeling disruption of epithelial barrier and tight juncitonsl. oranoid can be stained and imaged, then analyzred using 2D max projects or true 3D object analysis depending on your study design. Data quality was improved even further by selecting By selecting organoids based on siee, shape, and fluorecence usig nthe Celk Handler priort ot hte screen.

Unique Questions. Unique Solutions.