Neurobiology

Phenovista routinely offers services with co-cultures of neurons and astrocytes, 3D microBrains and most recently, the addition of human iPS-derived microglia to enable neuroinflammation studies.

Objective

Measure the rate of neurites extending from neurons.

Design

Human iPS-derived neurons (subtypes available such as Glut, Dopa and GABA as well as other species) plated then fixed at discrete timepoints (from 1 to 48hrs) then stained for viable nuclei and Tuj1 (neurite marker).

Viable cell and neurite network analysis is performed.

Advantages

Very flexible method enables a variety of timepoints to be collected, also very amenable to multiplexing to add ancillary markers related to mitochondrial health, ER stress and autophagy.

Many other markers are also supported.

Can be used to compare healthy vs disease models +/- stressors.

CNS/Neurobiology: Neurite Outgrowth

neurite_outgrowth-d

CNS/Neurobiology: Glutamate Toxicity

glutamate_toxicity

Objective

Assess neuronal toxicity in response to glutamate application.

Design

Human iPS-derived glutaminergic neurons alone and co-cultured with human iPS-derived astrocytes, glutamate applied and viability and cell health of neurons measured 72 hours later.

Advantages

More cost-effective and scalable than comparable in vivo approaches.

Objective

Profile marker panels and their disease-dependent differential response to stressors.

Design

Healthy and disease (e.g. Rett, Alzheimers, FTD etc) model human iPS-derived neurons (subtypes available such as Glut, Dopa and GABA as well as other species) plated then fixed at discrete timepoints after application of dose-response treatments with metabolic stressors such as FCCP, rotenone etc.

Advantages

Very flexible method enables a variety of markers to be explored and specific readouts created.

CNS/Neurobiology: Disease profiling

synapse_formation-c
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