MEASURING COLOCALIZATION OF PRE- AND POST-SYNAPTIC PROTEINS IN NEURONS
In the simple schematic example shown to the right, neurons are incubated for a period of time to promote neurite outgrowth and formation of synapses
Pre-synaptic markers such as Synaptophysin (green) and post-synaptic markers like PSD95 (red) can be detected with specific antibodies.
High resolution imaging and specialized 384 multi-well plates enable imaging of these micron-level structures.
Analysis of synapse formation uses colocalization (yellow) of pre- and post-synaptic markers constrained by a third, neurite marker (white).