USING IMAGING TO ANALYZE CELLULAR SUBPOPULATIONS
In the simple schematic example shown to the right, a relatively homogeneous baseline expression level of the protein of interest (depicted in green) exists within the untreated population.
Upon treatment, the expression level within each cell changes and two subpopulations arise (highlighted in yellow). The upper panel shows that a subpopulation with higher than baseline expression emerges. The lower panel shows that some cells within the population express lower levels of the protein depicted in green.
In all cases, the protein of interest remains cytoplasmic and diffusely distributed.