FIVE STEPS TO PHENOTYPIC DATA FROM AUTOMATED QUANTITATIVE IMAGING
Using a simplified example shown in the schematic to the right, two conditions are simulated; a control and treated phenotype.
In the control condition puncta are formed by the protein marker in both the cytoplasmic and nuclear compartments.
In the treated condition, the number of cytoplasmic puncta are increased while nuclear puncta are diminished.
Simplified example data for cell-level measurements is shown far right. Several different measurements are reported that capture aspects of intensity, area, object count, location and texture.
The final step is determining which output features are the most reliable for use in a quantitative assay.