MEASURING DISTRIBUTION OF PROTEINS ON THE PLASMA MEMBRANE

In the simple schematic example shown to the right, a cytoplasmic protein depicted in green translocates to the plasma membrane after treatment (bottom panel).

Similarly, treatment of the same cell population with a different stimulus leads to a change in distribution of the plasma membrane localized protein from diffuse to punctate.

This very simplistic example can be extended to measure the distribution of 4-5 different proteins (each detected via specific antibodies labeled with different color fluorescent tags) across multiple subcellular compartments AND formation of punct within each of those compartments.

Can this approach be extended to measure cell-cell junctions?
plasmamembranepuncta
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