SINGLE CELLS KINETIC MEASUREMENTS

Each cell can be identified via masking of either the nucleus or a cytoplasmic dye. From this signal, the XY centroid can be calculated for each cell. Using the XY centroid, other meta-data calculated for each cell over a series of time-points can be associated with a single cell.

This time-series of data can then be used to calculate kinetic properties for each cell. Kinetic properties might include the rate at which specific protein levels increase in the cytoplasm after treatment with an agonist compound.

How can these kinetic profiles be grouped?
kineticanalysis2b
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