WHAT ARE SOME OF THE CHALLENGES ASSOCIATED WITH PHENOTYPIC ASSAYS?
3D cultures are increasingly of interest to drug discovery scientists studying tumor microenvironments, neurospheres, gut organoids, stem cell colonies, blood vessels, and model organisms such as C.elegans and Zebrafish. Until recently, the majority of automated fluorescence microscopes (a.k.a. high content screening instruments) used epifluorescence or widefield ilumination so were not well-suited to thick samples. At Phenovista we have the latest confocal equipped HCS instrumentation (CX-7, ThermoScientific) that enables us to perform 3D imaging in 384 well plate format.
Phenotypic assays that use imaging, specifically automated quantitative fluorescence microscopy or high content screening (HCS), bring their own set of challenges. We’ve tried to address how we tackle these challenges but if you have other concerns please contact us.