MEASURING SUBCELLULAR DISTRIBUTION OF PROTEINS
In the simple schematic example shown to the right, a cytoplasmic protein depicted in green translocates to the nucleus after treatment (top panel). This type of phenomenon is common for signaling and transcription factors. Nuclear labeling via application of Hoechst (for clarity, Hoechst labeling depicted in blue in similar schematics is not shown in this example).
Similarly, treatment of the same cell population with a different stimulus leads to a change in distribution of the protein from the cytoplasm to the plasma membrane.
This approach can be extended to measure the distribution of 4-5 different proteins (each detected via specific antibodies labeled with different color fluorescent tags) across multiple subcellular compartments.