3D Biological Models, Spheroids and Organoids

PhenoVista allows clients to access the benefits of 3D culture work, without the acquisition of expertise or specialized equipment/culturing capabilities. 3D cell culture provides a more physiologically relevant environment that allows for:

  • Improved differentiation rates for stem cells

  • Drug safety and efficacy readouts not available in 2D cultures, e.g., longer-term hepatocyte cultures

  • 3D cultures provide better predictivity of drug resistance, as we are showing with PDX model work in vitro

Objective

Client was interested in identifying compounds that could protect against gut injury/disease and needed a 3D model system that recapitulated intestinal biology.

Design

DefiniGEN could provide a stem-cell derived organoid for testing both stresses and protective effects of client molecules.

Advantages

Organoids form intestinal lumen, allowing for modeling disruption of epithelial barrier and tight junctions.

Organoids can be stained and imaged using either the CX7 LZR or GE InCell6000 instruments and analyzed using 2D max projections or true 3D object analysis depending on your study design.

By selecting organoids based on size, shape and fluorescence using the Cell Handler, improving the robustness of downstream data.

3D Biology: Enteroids

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3D Biology: MicroBrains (Stemonix)

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Objective

Measure neuroprotection and impact on cell health in a complex organoid (astrocytes and multiple neuronal subtypes).

Design

Client was interested in evaluating protection against stresses, as well as whether certain cell types were more susceptible to stress (and protection).

Advantages

Complex and large organoids better recapitulate CNS biology.

Multicellular organoid structure allows examination of impact on individual cells (astrocytes vs. glutamate neurons vs GABA neurons).

Client could combine functional (FLIPR) assessment with imaging/structural readouts.

Client profiled a CNS-active library for comparison that allowed for determination of mechanism of action.

3D Biology: Ex-vivo Fragments (Yamaha)

Objective

Determine whether orthotopic PDX models could be converted to an in vitro assay for assessment of drug combinations prior to in vivo confirmation.

Design

PDX tumors were physically dissociated, and the Cell Handler was used to allocate tumor fragments precisely (size, shape, number per well) to 384-well plates for further testing.

Advantages

Converting PDX tumor models to in vitro allows for rapid assessment of multiple drug treatment/combination paradigms.

Immuno-oncology assets can also be tested with the addition of immune cells and stimulation.

3D Biology: Ex-vivo Fragments (Yamaha)

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