3D Biological Models, Spheroids and Organoids

PhenoVista allows clients to access the benefits of 3D culture work, without the acquisition of expertise or specialized equipment/culturing capabilities. 3D cell culture provides a more physiologically relevant environment that allows for:

  • Improved differentiation rates for stem cells

  • Drug safety and efficacy readouts not available in 2D cultures, e.g., longer-term hepatocyte cultures

  • 3D cultures provide better predictivity of drug resistance, as we are showing with PDX model work in vitro


Client was interested in identifying compounds that could protect against gut injury/disease and needed a 3D model system that recapitulated intestinal biology.


DefiniGEN could provide a stem-cell derived organoid for testing both stresses and protective effects of client molecules.


Organoids form intestinal lumen, allowing for modeling disruption of epithelial barrier and tight junctions.

Organoids can be stained and imaged using either the CX7 LZR or GE InCell6000 instruments and analyzed using 2D max projections or true 3D object analysis depending on your study design.

By selecting organoids based on size, shape and fluorescence using the Cell Handler, improving the robustness of downstream data.

3D Biology: Enteroids


3D Biology: MicroBrains (Stemonix)



Measure neuroprotection and impact on cell health in a complex organoid (astrocytes and multiple neuronal subtypes).


Client was interested in evaluating protection against stresses, as well as whether certain cell types were more susceptible to stress (and protection).


Complex and large organoids better recapitulate CNS biology.

Multicellular organoid structure allows examination of impact on individual cells (astrocytes vs. glutamate neurons vs GABA neurons).

Client could combine functional (FLIPR) assessment with imaging/structural readouts.

Client profiled a CNS-active library for comparison that allowed for determination of mechanism of action.

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