Imaging-based Phenotypic Screening is a extremely powerful and flexible approach to drug discovery and pre-clinical research.

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MEASURING COLOCALIZATION OF PROTEINS IN SUBCELLULAR STRUCTURES

In the simple schematic example shown to the right, a plasma membrane protein depicted in green is degraded in lysosomes (depicted in red) under certain conditions.

Colocalization is a quantitative measurement using pixel intensities of two (sometimes three) labels. In this example the degree of colocalization is measured between the protein depicted in green and the lysosomal marker depicted in red.

Perfect colocalization is rare but this approach can be used to assess the degree of association between pairs of proteins or markers.

Colocalization can also be constrained by a third marker. For example, analysis of two transcription factors constrained by the nuclear mask Hoechst.

What about quantifying micron-level structures like synapses? >>>

colocalization

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