Imaging-based Phenotypic Screening is a extremely powerful and flexible approach to drug discovery and pre-clinical research.
Learn how imaging-based phenotypic assays work and what kind of information you can expect from working with us.
FIVE STEPS TO PHENOTYPIC DATA FROM AUTOMATED QUANTITATIVE IMAGING
In the simple schematic example shown to the right, a two channel assay is performed.
The first channel is occupied by the nuclear marker Hoechst. This channel is used primarily to focus the imaging system in the correct Z plane where the cells are located. The Hoechst channel is also used to count cells and quantify other nuclear properties such as size, shape and texture – these features can be used to detect cell cycle, toxicity and stress.
The second channel is depicted in green and simulates a cytoplasmic protein that forms puncta in the cytoplasm and nuclear compartments.